The plant U‐box (PUB) protein functions as an E3 ligase to poly‐ubiquitinate a target protein for its degradation or post‐translational modification. Here, Nike Shox Nz Trainers we report functional roles for OsPUB15, which encodes a cytosolic U‐box protein in the class‐II PUB family. Self‐ubiquitination assays showed Cheap Air Force Uk that bacterially expressed MBP‐OsPUB15 protein has E3 ubiquitin ligase activity. A T‐DNA insertional mutation in OsPUB15 caused severe growth retardation and a seedling‐lethal Air Force One phenotype.
Mutant seeds did not produce primary roots, and their shoot Nike Shox Shoes For Women development was significantly delayed. Transgenic plants expressing the OsPUB15 antisense transcript phenocopied these mutant characters. The abnormal phenotypes were partially rescued by two antioxidants, catechin and ascorbic acid. Germinating… seeds in the dark also recovered the rootless defect.
Levels of H2O2 and oxidized Nike Air Force Uk proteins were higher in the Air Max 2011 knock‐out mutant compared with the wild type. OsPUB15 transcript levels were increased upon H2O2, salt and drought stresses; plants overexpressing the gene grew better than the wild type under high salinity. These results indicate that PUB15 is a regulator that reduces reactive oxygen species (ROS) stress and cell death.